osnaDocs: Nonlinear optical properties of modified Bacteriorhodopsins

Nonlinear optical properties of modified Bacteriorhodopsins

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https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2008020119

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DC Field	Value	Language
dc.contributor.advisor	Prof. Dr. Heinz-Jürgen Steinhoff
dc.creator	Krasnaberski, Aliaksei
dc.date.accessioned	2010-01-30T14:36:46Z
dc.date.available	2010-01-30T14:36:46Z
dc.date.issued	2008-01-30T18:18:49Z
dc.date.submitted	2008-01-30T18:18:49Z
dc.identifier.uri	https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2008020119	-
dc.description.abstract	Recent years have shown a dramatic growth of research activity in searching of materials that exhibit nonlinear optical (NLO) behaviours. Later investigations have shown that molecule-based organic materials and biomaterials possess advantages in NLO characteristics. This thesis is devoted to the theoretical and experimental study of the second-order nonlinear optical properties of native and modified bacteriorhodopsins. A hybrid quantum mechanical/molecular mechanical-configuration interaction (QM/MM-CI) method has been used to determine the nonlinear behaviour of modified BR. The calculated structures are the ground state and M1 state of wild type BR, several BR variants, the BR mutants W86F, W189F, W182F, W138C, Y185F, and Y83F as well as a photoreceptor NpSRII. The first hyperpolarizability of two available BR mutants W86F and D85C has been determined by means of the HRS technique. The experimentally determined first hyperpolarizability value for the mutant W86F is in good agreement with that obtained theoretically. Simultaneously with the time-resolved absorption spectroscopy measurements, the time-dependent HRS measurements were performed. Changes in the HRS signal during the photocycle were detected. The kinetics of time-resolved HRS and flash spectroscopy measurements of BR and the BR mutants T46C, R225C, R227C, D96C, and D85C were compared. The obtained kinetic constant are comparable with those determined by means of absorption spectroscopy. However, an unexpected decrease in the HRS intensity during the M to N transition was observed. The program "MultiFit" (written in the Delphi environment) as well as data acquisition software (written in the C environment) can be found online at: http://repositorium.uni-osnabrueck.de/bitstream/urn:nbn:de:gbv:700-2008020119/4/MultiFit.zip and: http://repositorium.uni-osnabrueck.de/bitstream/urn:nbn:de:gbv:700-2008020119/5/TRAS.zip	eng
dc.language.iso	eng
dc.subject	Bacteriorhodopsin
dc.subject	first hyperpolarizability
dc.subject	hybrid quantum mechanical/molecular mechanical
dc.subject	Hyper-Raleigh scattering technique
dc.subject.ddc	570 - Biowissenschaften, Biologie	ger
dc.title	Nonlinear optical properties of modified Bacteriorhodopsins	eng
dc.type	Dissertation oder Habilitation [doctoralThesis]	-
thesis.location	Osnabrück	-
thesis.institution	Universität	-
thesis.type	Dissertation [thesis.doctoral]	-
thesis.date	2008-01-28T12:00:00Z	-
elib.elibid	766	-
elib.marc.edt	jost	-
elib.dct.accessRights	a	-
elib.dct.created	2008-01-30T16:56:20Z	-
elib.dct.modified	2008-01-30T18:18:49Z	-
dc.contributor.referee	Prof. Dr. Klaus Betzler
dc.subject.dnb	29 - Physik, Astronomie	ger
vCard.ORG	FB4	ger
Appears in Collections:	FB06 - E-Dissertationen

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File	Description	Size	Format
E-Diss766_thesis.pdf	Präsentationsformat	2,14 MB	Adobe PDF	E-Diss766_thesis.pdf View/Open
MultiFit.zip		421,27 kB	ZIP	MultiFit.zip View/Open
TRAS.zip		857,66 kB	ZIP	TRAS.zip View/Open

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