Title:	Enzyme assays with supramolecular chemosensors – the label-free approach
Authors:	Nilama, Mohamed Hennig, Andreas
ORCID of the author:	https://orcid.org/0000-0003-0444-5923
Abstract:	Enzyme activity measurements are essential for many research areas, e.g., for the identification of inhibitors in drug discovery, in bioengineering of enzyme mutants for biotechnological applications, or in bioanalytical chemistry as parts of biosensors. In particular in high-throughput screening (HTS), sensitive optical detection is most preferred and numerous absorption and fluorescence spectroscopy-based enzyme assays have been developed, which most frequently require time-consuming fluorescent labelling that may interfere with biological recognition. The use of supramolecular chemosensors, which can specifically signal analytes with fluorescence-based read-out methods, affords an attractive and label-free alternative to more established enzyme assays. We provide herein a comprehensive review that summarizes the current state-of-the-art of supramolecular enzyme assays ranging from early examples with covalent chemosensors to the most recent applications of supramolecular tandem enzyme assays, which utilize common and often commercially available combinations of macrocyclic host molecules (e.g. cyclodextrins, calixarenes, and cucurbiturils) and fluorescent dyes as self-assembled reporter pairs for assaying enzyme activity.
Citations:	Nilam, M., & Hennig, A. (2022): Enzyme assays with supramolecular chemosensors – the label-free approach. RSC Advances, 12(17), 10725-10748.
URL:	https://doi.org/10.48693/310 https://osnadocs.ub.uni-osnabrueck.de/handle/ds-202305038851
Subject Keywords:	supramolecular chemosensors; label-free approach; enzyme activity; fluorogenic; Fluoroimmunoassays
Issue Date:	6-Apr-2022
License name:	Attribution 4.0 International
License url:	http://creativecommons.org/licenses/by/4.0/
Type of publication:	Einzelbeitrag in einer wissenschaftlichen Zeitschrift [Article]
Appears in Collections:	FB05 - Hochschulschriften Open-Access-Publikationsfonds

This item is licensed under a Creative Commons License